Redox Research Center

Japan’s first laboratory specialized in redox (oxidation and reduction) analysis.

The Redox Research Center specializes in analysis of oxidation and reduction – redox in human body with our own reagents. Our tests facilitate large batch of samples from our clients such as researchers, athlete coaches, and more who seek to further acquire accuracy in researches and improve in conditioning of athletes.
See below to find out more about our tests, Redox analysis and Lipidomics analysis.

Redox analysis

*This test is for research purposes and only limited to requests in Japan.

We accept serum and plasma samples and perform d-ROMs, BAP, and OXY adsorption tests on behalf of our clients who are not equipped with the necessary devices, such as FRAS and FREE.

The facility is equipped with a biochemical analyzer specially designed for d-ROMs, BAP, and OXY adsorption tests to measure a large number of samples in a short time. As a reference, the tests will take less than 1 hour for 100 samples.

Test categories

Test		Code	Sample	Unit
Test		（method）	（storage）	（range）
Oxidation marker	d-ROMs	DI-d01	serum / heparin plasma	U.CARR
		(colorimetry)	(*1 year)	（40-1000）
		Remarks: The most effective method to identify the initial oxidation state.The total oxidation degree in the serum and plasma of humans, animals, fish, etc. is quantified by the color reaction of DEPPD. 1U.CARR has an oxidizing capacity equivalent to 0.08mgH202 / dL. Note:The amount converted to H202 is not pre-existent in blood.
Antioxidant marker	BAP	DI-b01	serum / heparin plasma	μmol/l
		(colorimetry)	(*6 months)	（500-6000）
		Remarks: Measures the antioxidant capacity of endogenous antioxidants such as albumin, bilirubin, reduced glutathione, and uric acid, and exogenous antioxidants such as vitamins C / E and polyphenols from the reducing power of trivalent iron ions. Serum, plasma and saliva of human, animals, fish, etc. can be measured.
	OXY adsorption	DI-oxy01	serum / heparin plasma	μmol/ml
		(colorimetry)	(*4 months)	（80-500）
		Remarks: Assessing the overall antioxidant level based on the ability to eliminate hypochlorous acid (HClO), the most powerful reactive oxygen species created when leukocytes in vivo that attack foreign substances. It is also effective for measuring the antioxidant level of foods such as juice, vegetables, and wine.

*Samples are stored below -20℃.

Lipidomics (fatty acids) analysis

*This test is for research purposes and only limited to requests in Japan.

This test comprehensively analyzes the intracellular distribution of a total of 22 fatty acids, including omega-3 fatty acids (such as EPA and DHA), omega-6 fatty acids, saturated fatty acids, monounsaturated fatty acids, and bad trans-fatty acids, of which describes the balance of fatty acids in the body.

The test results can be a guide to consuming the right fatty acids, so not only those who are suffering from lifestyle-related diseases such as dyslipidemia and diabetes, and those suffering from dementia and developmental disorders, this test can be used widely for health maintenance.

The balance of fatty acids is also important to balancing the redox function in the body. The results from this test will be an effective tool in dietary guidance to patients.

Reference: interpretation of results

SFA / MUFA ratio

Saturated fatty acids (SFA) are usually solid at room temperature. High in fats of beef, pork, lard, lamb, and egg yolk, and also found in tropical vegetable oils such as coconut oil, palm kernel oil, cocoa butter and palm oil.
Main roles: raises blood cholesterol levels and increases the risk of heart disease.

Monounsaturated fatty acids (MUFA) are liquid at room temperature. Examples of foods high in MUFA content include olives, peanuts, almonds, cashews, macadamia nuts, and avocados. Main role: Decrease blood cholesterol.

Omega-3 Index

The omega-3 index is a measure of the amount of omega-3 fatty acids EPA + DHA in red blood cells which is a measure of cardiovascular health. It has now also been discovered that these omega-3 fatty acids keep cells young for a longer period of time. Low omega-3 fatty acid levels are associated with increased risk of fatal heart attack, depression and dementia.
Main roles: Anti-inflammatory, anti-allergic, vasodilator.

Trans Fat Index

Although small amounts of trans-fatty acids are found “naturally” in foods such as full fat dairy products and red meat, the majority (80-90%) of trans-fatty acids result from “partial hydrogenation” of liquid vegetable oils. The consumption of these “industrially produced trans-fatty acids” is associated with a high risk of heart attack.
Main roles: Obesity, decrease in immune function, atherosclerosis, increase in the risk of heart attack

AA / EPA ratio

There is a ratio of two important fatty acids in the blood that indicates the level of inflammation in the cells of the body. These fatty acids are arachidonic acid (AA), an omega-6 fatty acid, and eicosapentaenoic acid (EPA), an omega-3 fatty acid. AA produces pro-inflammatory hormones that over-accelerate chronic diseases. EPA produces a potent anti-inflammatory hormones that help resolve inflammation.

AA/EPA ratio	Inflammation in cells	Future health level
1.5-3.0	Low	Healthy
3.0-6.0	Slightly low	Healthy
7.0-15.0	Moderate	Moderate
Above 15.0	Moderate	Unhealthy

Benefits of lipidomics analysis

An indicator of dietary improvement
Guide in health management
Improvement in lifestyle-related diseases
Prevention of dementia
Improvement in sport performance

Growing importance of lipidomics

Fatty acids are an important source of energy in the body and are constituents of cell membranes. Prostaglandins derived from omega-6 fatty acids (arachidonic acid) are involved in regulating immune function, digestive function, and inflammation, and leukotrienes are involved in allergic and inflammatory reactions. Omega-3 fatty acids have anti-inflammatory and anti-allergic effects and are closely related to the physiological role of living organisms. In addition, it plays an important role in signaling between cells, regulating hormone balance, and assisting in vitamin absorption.

Analyzing lipid molecules in vivo is thought to be useful for understanding various life activities, elucidating pathophysiological conditions, and analyzing therapeutic effects.

Analysis request process

Blood sample preparation to receiving test results

STEP 1: Receive a kit from Redox Research Center
STEP 2: Prepare sample with the kit
(drop blood samples on 5 circled spots)
STEP 3: Send the kit to Redox Research Center
STEP 4: Receive a report in 3-4 weeks later via mail

*Analysis requests are only limited to Japan.