An easy-to-use reagent kit to measure oxidative stress from blood samples.

As reactive oxygen and free radicals, the cause of oxidative stress, are known to have a short shelf life and are highly reactive, its measurement has been a challenge, especially in clinical applications. The d-ROMs test does not directly measure reactive oxygen and free radicals, but rather quantifies the metabolite ROOH to evaluate oxidative stress. This makes applications in clinical research as well as in various other fields (food, sports, etc.) possible.

How does it work?

It identifies total peroxides, including hydroperoxides (ROOH), which are metabolites generated when reactive oxygen and free radicals oxidize the body’s constituent such as lipids, proteins, amino acids, and nucleic acids. The sample is mixed with 4.8 PH acetate buffer and peroxide is converted back into radicals in the presence of iron ions. A color solution (N, N-Diethyl-p-phenylenediamine) that reacts with the radical is then added, and the concentration is measured. The unit of measurement is “CARR U”. (1 CARR U = 0.08 mg H2O2 / dL)

Samples and storage

The sample should be serum / heparin plasma (*EDTA and citrate samples are not viable). Samples can last for 1 year when it is stored at -20 °C or lower.

Reagent classification

Device Reagent Code
FREE Carrio Duo 50 kits of d-ROMs test DI-003b
FREE carpe diem 50 kits of d-ROMs test DI-003b
FRAS4 50 U-kits of d-ROMs test  DI-001b


Lowest detectable limit 11 UCARR
Lowest quantifiable limit 40 UCARR
Measurement range 40-1000 UCARR
Simultaneous reproducibility
n= 20
300.3 6.20 2.07
n= 20
295.0 5.28 1.79
Coefficient of variation CV % = 1.72

Coefficient of determination
(Correlation between absorbance and analyte concentration)

R2 = 0.9981
Recovery test (%) 90-103 %

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