d-ROMs test

An easy-to-use reagent kit to measure oxidative stress from blood samples.

As reactive oxygen and free radicals, the cause of oxidative stress, are known to have a short shelf life and are highly reactive, its measurement has been a challenge, especially in clinical applications. The d-ROMs test does not directly measure reactive oxygen and free radicals, but rather quantifies the metabolite ROOH to evaluate oxidative stress. This makes applications in clinical research as well as in various other fields (food, sports, etc.) possible.

How does it work?

It identifies total peroxides, including hydroperoxides (ROOH), which are metabolites generated when reactive oxygen and free radicals oxidize the body’s constituent such as lipids, proteins, amino acids, and nucleic acids. The sample is mixed with 4.8 PH acetate buffer and peroxide is converted back into radicals in the presence of iron ions. A color solution (N, N-Diethyl-p-phenylenediamine) that reacts with the radical is then added, and the concentration is measured. The unit of measurement is “CARR U”. (1 CARR U = 0.08 mg H₂O₂ / dL)

Samples and storage

The sample should be serum / heparin plasma (*EDTA and citrate samples are not viable). Samples can last for 1 year when it is stored at -20 °C or lower.

Reagent classification

Device	Reagent	Code
FREE Carrio Duo	50 kits of d-ROMs test	DI-003b
FREE carpe diem	50 kits of d-ROMs test	DI-003b
FRAS4	50 U-kits of d-ROMs test	DI-001b

Performance

Lowest detectable limit	11 UCARR
Lowest quantifiable limit	40 UCARR
Measurement range	40-1000 UCARR
Simultaneous reproducibility n= 20	Mean (UCARR)	DS	CV%
Simultaneous reproducibility n= 20	300.3	6.20	2.07
Reproducibility n= 20	Mean (UCARR)	DS	CV%
Reproducibility n= 20	295.0	5.28	1.79
Coefficient of variation	CV % = 1.72
Coefficient of determination （Correlation between absorbance and analyte concentration）	R² = 0.9981
Recovery test (%)	90-103 %