OXY adsorbent test is a reagent kit to evaluate the overall antioxidant level with high accuracy.

Reactive oxygen and free radicals, the cause of oxidative stress, are constantly produced by metabolism and immune activities in the body. In this OXY adsorption test, hypochlorite (HClO), one of the powerful reactive oxygen species emitted by leukocytes, is used to quantify antioxidant level in a body more accurately. In addition, water-soluble samples such as foods, beverages and supplements, can also be measured to quantify its antioxidant level. With one reagent, the antioxidant level of the consumed items and its effect in the body can be measured through a blood sample. Also, by combining it with d-ROMs test, etc., the range of applications can be explored further.

How does it work?

A sample is mixed with the reagent, hypochlorous acid (HClO), and the sample undergoes an antioxidant reaction against the reagent. After a specific amount of time, a coloring solution (N, N-Diethyl-p-phenylenediamine) is added to react with the remaining hypochlorous acid to cause a red coloring reaction (A-NH2 → [A-NH2・]+).

Like BAP test, the OXY adsorbent test measures the antioxidant level of endogenous antioxidants such as albumin, bilirubin, reduced glutathione, and uric acid, and exogenous antioxidants such as vitamin C / E and polyphenol, and it can also quantify antioxidants called shock-adsorbents (e.g. mucopolysaccharides, amino acids, proteins, etc.) that can “suppress” the oxidative action of ROS, which allows a total evaluation of antioxidant level.

Samples and storage

The sample should be serum / heparin plasma (*EDTA and citrate samples are not viable). Samples can last for 4 months when it is stored at -20 °C or lower.

Water-soluble liquids (fruit, vegetables, wine, alcohol, juices, etc.), tablets and powder samples can be measured after diluted with distilled water.

*Reference of food antioxidant level measured in Italy.

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